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...The A and B form of NBS1 mRNA: a potential mechanism of conformational change in the pathogenesis of Nijmegen breakage syndrome. Nijmegen breakage syndrome (NBS) is a rare, recessively inherited cancer predisposition disorder characterized by microcephaly, immunodeficiency, and predisposition to leukemia and solid tumors. The affected genes include a 60 kDa subunit of the Mre11-Rad50-Nbs1 (MRN) complex and a protein known as NBS1. NBS1, a nuclear phosphoprotein, contains a 3'-untranslated region (3'UTR) in the form of a short and long mRNA. Short and long 3'UTRs have different lengths of poly(A) tails, and have been reported to exist as A and B isoforms. We report here that both A and B mRNA isoforms exist in multiple types of human cells. In A form-expressing cells, both the long and short 3'UTR exist, while in B form-expressing cells, only the long 3'UTR exists. The size of 3'UTR-long poly(A) tail was observed to change in the presence of RNase H, a component of the ribonucleoprotein complex, suggesting that the 3'UTR of B form mRNA is directly linked to poly(A) tails. However, the elongation of 3'UTR-long poly(A) tail was not observed in the presence of protein synthesis inhibitor. We propose that the length of 3'UTR-long poly(A) tail changes in the presence of protein synthesis inhibitor, which induces the conformational change in the long form of NBS1 mRNA. This result suggests a mechanism of conformational change in the pathogenesis of NBS.Q: How to use ARMarker in MKMapView I want to add ARMarker in my MKMapView. I tried this example: But, I can't import class "CoreLocation" I want to use marker in my mapview. A: I found the solution and I post here. In your project, select the project target and add Core Location framework. Create a ViewController and add below code in ViewDidLoad method. -(void)view


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